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    Effects of Nigella sativa oil on immune mechanisms in rainbow trout, Oncorhynchus mykiss
    (2024) ISPIR, ÜNAL; ÖZCAN, Mikail; KIRICI, Muammer
    The purpose of this study was to evaluate the efficacy of Nigella sativa oil on serum responses in rainbow trout. Fish were injected intraperitoneally (i.p.) with 1% and 10% doses of NSO. Serum bactericidal activity, lysozyme activity, myeloperoxidase, total protein and total immunoglobulin levels were analyzed 3, 7, 14 and 21 days after injection. The same procedure was performed in a control group. There were signif-icant changes in all immunological parameters among the treatment groups except for total protein and immunoglobulin levels. In conclusion, the present study showed that the immune parameters of rainbow trout were increased. Therefore, injection of Ni-gella sativa oil leads to a positive effect on the immune response of rainbow trout.
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    Fatty Acid Composition in Yersinia ruckeri Strains Isolated from Rainbow Trout Farms
    (2021) ISPIR, ÜNAL; ÖZCAN, Mikail; KIRICI, Muammer
    In this study, a total of 33 Yersinia ruckeri isolates were obtained from rainbow trout farms in the five different region of Turkey. We determined to changes in fatty acids group of the strains. All the isolates were identified as Y. ruckeri based on colonical, cellular morphology and biochemical characters. All Y. ruckeri 8 major fatty acids, including 12:0 (Lauric acid), 14:0 (Myristic acid), 16:0 (Palmitic acid), 16:1n-7 (Palmitoleic acid), 17:1 (Heptadecanoic acid), 18:1n-9c (Oleic acid), 18:2n-6c (Linoleic acid), 18:3n-3c (Alfa-linoleic acid). Compared to isolates biochemical property, there was no difference between fatty acids and biochemical characteristics. The results of this study showed that the fatty acids composition of Y. ruckeri isolated strains from rainbow trout farms in Turkey is on a large scale homogenous.