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    Changes of the Expressions of Orphan and gon-ADAMTS in Chondrosarcoma Cells
    (Akad Doktorlar Yayinevi, 2015) Isik, Bunyamin; Erdemli, Haci K.; Comertoglu, Ismail; Akyol, Sumeyya; Firat, Ridvan; Kaya, Mehmet; Akyol, Omer
    Some of A disintegrin and metalloproteinase with thrombospondin motifs (ADAMTS) enzymes have been suggested to facilitate invasion and metastasis in cancer. ADAMTS20 is called gon-ADAMTS and ADAMTS10 and -17 are called orphan ADAMTSs. ADAMTS20 degrades versican and aggrecan in extracellular matrix. We aimed to investigate the effects of insulin on ADAMTS10,-17 and -20 in OUMS-27 chondrosarcoma cells. OUMS-27 cells were cultured in Dulbecco's modified Eagle' medium (DMEM) containing 10 mu g/mL insulin. The medium was changed every other day up to 11th day. Cells were harvested at 1, 3, 7, and 11th days and RNA isolation was performed at appropriate times according to study setup. The levels of RNA expression of ADAMTS10,-17 and -20 were estimated by qRT-PCR using appropriate primers. ADAMTS10 mRNA expression gradually decreased within 7 days after insulin induction compared to control group. There was a significant difference between control and Day 7 groups (p=0.021) as well as Day 1 and Day 7 groups (p=0.028). ADAMTS17 mRNA expression increased right after insulin induction at day 1 compared to control group and protected its high levels throughout insulin application. The most evident and statistically significant increase in mRNA concentration was observed at day 7 after insulin induction (p=0.014). Our results demonstrated that ADAMTS10,-17 and -20 might have a role in cancer progression. Although functions of ADAMTS10 and -17 are not known, their expression levels have changed in chondrosarcoma cell line. Further studies are needed to characterize chondrosarcoma cells because of the possible association of cancer progression and ADAMTS proteins.
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    Factors affecting daily instrumental activities of the elderly
    (Tubitak Scientific & Technological Research Council Turkey, 2015) Sahin, Ayfer; Tekin, Oguz; Cebeci, Sevsen; Isik, Bunyamin; Ozkara, Adem; Kahveci, Rabia; Canbal, Metin
    Background/aim: In order to determine elderly people's capabilities in daily activities, we evaluated the factors that may affect their daily instrumental activities. Materials and methods: We performed an observational, analytical, and cross-sectional study on 101 patients above 60 years of age in 2008 and 2009. We applied the Lawton Instrumental Activity of Daily Living (IADL) scale, the Standardized Mini-Mental State Examination (SMMSE), and the Geriatric Depression Scale (GDS) during one-on-one interviews with the patients. Demographic data and disability levels were also recorded. These data were used to evaluate the possible effects of factors on the IADL scale. Results: Statistical analyses indicated that total scores of instrumental activities are affected negatively by increased age, female sex, and literacy (P < 0.001, P = 0.005, and P = 0.021), whereas scores are affected positively by educational level (P = 0.047). Conclusion: Our findings suggest that increased age, sex, literacy, and education levels influence elderly people's daily instrumental activities. Daily functional activities and factors influencing these activities should be determined in order to increase elderly people's quality of life and independence. It is important to evaluate elderly people's capabilities in daily activities.
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    Future opportunities in preventing ototoxicity: Caffeic acid phenethyl ester may be a candidate
    (Spandidos Publ Ltd, 2015) Akyol, Sumeyya; Isik, Bunyamin; Altuntas, Aynur; Erden, Gonul; Cakmak, Ozlem; Kursunlu, Fatih; Adam, Bahattin
    Caffeic acid phenethyl ester (CAPE) is an important active component of propolis, which is derived from honeybee hives. It has received increasing attention in a variety of medical and pharmaceutical research, due to its anti-oxidant, antiproliferative, anti-inflammatory, antiviral and antifungal activity, in addition to its antineoplastic properties. Besides the use of CAPE as an antioxidant and anti-inflammatory agent in a number of in vivo studies of ear disease, its beneficial effects have been reported in the treatment of cancer, arthritis, allergies, heart disease, diabetes, kidney disease, liver disease and neurological disease. CAPE influences a number of biochemical pathways, as well as several targets involved in ear diseases, in particular, in ototoxicity. The protective effects of CAPE in ototoxicity, which may be induced by a number factors, including lipopolysaccharides, hydrogen peroxide and streptomycin, are evaluated and discussed in the present review.
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    Reflexological Therapy Induces a State of Balance in Autonomic Nervous System
    (Canadian Soc Clinical Investigation, 2015) Isik, Bunyamin; Aydin, Duygu; Arslan, Muzeyyen; Dane, Senol
    Purpose: Reflexology activate all body systems physically, mentally and emotionally by relieving pain and relaxing the body, in a manner similar to acupuncture and cupping therapies. The aim of this study was to examine the effect of reflexological therapy on heart rate variability (HRV) parameters in a healthy population. Methods: Participants were twenty-six healthy subjects (8 women, 18 men, median age=32.77 years, SD=8.04). All reflexology procedures were applied by a reflexology practitioner. The reflexology practitioner used thumbs and fingers to apply appropriate pressure to reflexology points, especially the heart point in both feet. The recording ECG was applied 1 hour before and 1 hour after reflexological therapy. Subjects rested for 10 minutes without recording ECG in order to stabilize autonomic parameters. The digital signals were then transferred to a laptop and analyzed using LabChart (R) software (MLS310/7 HRV Module). Results: Almost all HRV parameters increased and heart (pulse) rate and LF/HF ratio decreased after reflexological therapy compared with before reflexological therapy in healthy persons. Conclusions: These results indicate for the first time in humans that reflexology might induce a state of balance between sympathetic and parasympathetic systems and might be helpful to prevent possible cardiac arrhythmias. Therefore, reflexology may be accepted as a complementary therapy method for many cardiac problems, especially tachycardia and other cardiac arrhythmias.