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Öğe A comparison of hair and serum trace elements in patients with Alzheimer disease and healthy participants(Tubitak Scientific & Technological Research Council Turkey, 2015) Koc, Emine Rabia; Ilhan, Atilla; Ayturk, Zubeyde; Acar, Burcu; Gurler, Mukaddes; Altuntas, Aynur; Karapirli, MustafaBackground/aim: To determine whether there was a difference between serum and hair trace elements' concentrations in patients with Alzheimer disease (AD) and healthy participants. Materials and methods: Hair and serum copper, selenium, zinc, magnesium, manganese, and iron levels were measured by inductively coupled plasma-mass spectrometry in patients with AD and healthy participants, and the obtained results were statistically compared. Results: The mean hair selenium and zinc levels of patients with AD were significantly lower than the levels found for control participants (P < 0.05). Patients with AD had significantly higher mean hair copper and manganese levels than the controls. There were no significant differences between AD patients and controls with respect to the hair iron and magnesium levels (P > 0.05). Hair and serum trace element (copper, selenium, zinc, magnesium, manganese, and iron) levels in patients with AD showed no significant difference according to mini mental test scores or sex (P > 0.05). Conclusion: Some trace element levels may change in patients with AD. Due to the more permanent status, the analysis of these element levels in hair might be superior to blood analysis.Öğe HAIR AND SERUM TRACE ELEMENT LEVELS IN ALZHEIMER'S DISEASE(Elsevier Science Inc, 2014) Koc, Emine R.; Ayturk, Zubeyde; Ilhan, Atilla; Acar, Burcu; Gurler, Mukaddes; Bodur, M. Said[Abstract Not Available]Öğe Hydrogen Peroxide-Induced Oxidative Damage in Human Chondrocytes: The Prophylactic Effects of Hypericum Perforatum Linn Extract on Deoxyribonucleic Acid Damage, Apoptosis and Matrix Remodeling by a Disintegrin-Like and Metalloproteinase With Thrombospondin Motifs Proteinases(Turkish League Against Rheumatism, 2014) Akyol, Sumeyya; Yukselten, Yunus; Cakmak, Ozlem; Ugurcu, Veli; Altuntas, Aynur; Gurler, Mukaddes; Akyol, OmerObjectives: This in vitro study aimed to examine the protective roles of Hypericum perforatum Linn (HPL) extract on cell viability, DNA damage, apoptosis and a disintegrin-like and metalloproteinase with thrombospondin motifs (ADAMTS) proteins in chondrocytes induced by hydrogen peroxide (H2O2), as a model of chondrocytes subjected to reactive oxygen species attack in rheumatoid arthritis and osteoarthritis. Materials and methods: Human chondrosarcoma cell line (OUMS-27) was used. Cells were incubated with different concentrations of methanolic extract (100, 400, and 750 mu g/ml) of HPL for 36 hours, and then treated with 0.7 mM H2O2 for two hours. Trypan blue was used for evaluation of cell viability, while DNA damage was evaluated by alkaline Comet assay. Caspase-1, ADAMTS5, ADAMTS9, and glyceraldehyde-3-phosphate dehydrogenase proteins were analyzed by Western blot. Results: In vitro H2O2 treatment decreased OUMS-27 cell viability. Cells pretreated with HPL at concentration of 400 mu g/mL were best protected from H2O2 toxicity. Compared to 100 mu g/ml concentration, pretreatment of cells with 750 or 400 mu g/ml of HPL generated more protection against H2O2-induced DNA damage. Hydrogen peroxide application to the cells led to a slight increase in Caspase-1 expression, which shows no apoptosis. The most prominent increase in Caspase-1 level was shown in cells treated with 400 mu g/ml of HPL extract. There was an increase in ADAMTS9 and a decrease in ADAMTS5 levels upon H2O2 administration. Pretreatment with HPL led to more decrease in ADAMTS5 level, indicating the protection of extracellular matrix attacked by these proteinases in cartilage tissue. Conclusion: It can be concluded that HPL has a potential to reverse the negative effects and processes induced by H2O2 in OUMS-27 cells and it can protect the surrounding cartilage area of chondrocytes from oxidative damage, which is suggested to be one of the main molecular factors accused for progression of rheumatoid arthritis and osteoarthritis.Öğe Simultaneous analysis of biochemical markers in vitreous humour and serum: a preliminary study on the effect of storage time at -20°C(Taylor & Francis Ltd, 2016) Gurler, Mukaddes; Ozturk, Gulfer; Kir, M. Ziya; Ginis, Zeynep; Erden, Gonul; Akyol, Sumeyya; Kaya, MehmetVitreous humour (VH) is a quite stable and well-protected biological fluid from post-mortem degradation and contamination, making it a usable specimen in forensic pathology. We aimed to analyse the biochemical markers in VH stored for one month at -20 degrees C and to compare them with those of serum samples of the same deceased people. To the best of our knowledge this is the first time this has been reported in the literature. Serum levels of sodium, potassium and calcium, phosphorous and VH levels of calcium, creatinine, and urea were not statistically different between fresh and stored samples. There was no significant difference in VH urea and creatinine levels whereas serum levels were significantly decreased by the 15th and 30th days. In conclusion, urea and creatinine levels in VH showed adequate stability after one month of storage at -20 degrees C, whereas large variations were seen in the stability of inorganic components such as Na, K, Mg, Cl, and P. Our results indicate the validity of using post-mortem VH samples for urea and creatinine but not for VH stored at -20 degrees C for a limited time frame. The differences in sample handling before being stored at -20 degrees C may introduce bias on some components of VH.
