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    A disintegrin-like metalloproteinase with thrombospondin MOTIF 8 expression analysis in OUMS-27 chondrosarcoma cells before and after insulin administration
    (National Institute of Science Communication and Information Resources (NISCAIR) Dr. K. S. Krishnan Marg (Near Pusa Gate) New Delhi 110-012, 2016) Erden, Gönül; Akyol, Sumeyya; Com?ertog?u, İsmail; Altuntaş, Aynur; Çakmak, Özlem; Uğurcu, Veli; Yükselten, Yunus
    A disintegrin-like and metalloproteinase with thrombospondin motif 8 (ADAMTS8) is a secreted protease with anti-angiogenic properties. It inhibits vascular endothelial growth factor (VEGF) induced angiogenesis and suppresses fibroblast growth factor 2 (FGF-2) induced vascularization. Angiogenesis and extracellular matrix degradation are the key events in tumor progression, and ADAMTS8 is also known to be a member of the aggrecanases family. In the present study, we investigated the expression levels of ADAMTS8 in chondrosarcoma cells to elucidate the effect of insulin on the tumor cells in terms of ADAMTS production. The OUMS-27 cells were cultured and separately exposed to 10 ?mol/mL insulin up to 11 days in Dulbecco’s modified Eagle’s medium. After specific time limits (days 1, 3, 7, and 11), the culture was terminated and RNA was isolated using TRIzol reagent and converted to cDNA. The expression levels of ADAMTS8 were evaluated by qRT-PCR. The ADAMTS8 expression in OUMS-27 cells exhibited about 4-fold decrease following insulin treatment on day 11. Statistically significant differences were noted between the control and day 1 (P = 0.008), day 7 (P = 0.047) and day 11 (P = 0.008) groups. The effect of insulin on chondrosarcoma cells in terms of ADAMTS8 expression has not been reported earlier. The decrease in ADAMTS8 expression could be considered as a novel finding that has the potential to explain some pathophysiological mechanisms of tumor cells. Furthermore, the finding could also shed some light on the relationships between matrix degradation and insulin treatment in vitro. © 2017 Elsevier B.V., All rights reserved.
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    Evaluation of serum vitamin D levels in elderly patients with subclinical hypothyroidism
    (Ondokuz Mayis Universitesi Samsun 55139, 2014) Uçar, Fatma; Akyol, Sumeyya; Öztürk, Gülfer; Giniş, Zeynep; Erden, Gönül; Taslipinara, Mine Yavuz; Arzuhala, Abdullah Ercan
    Increasing numbers of researches have indicated the relationship between serum vitamin D level and several autoimmune diseases, because it has important roles in immune regulation. However, the role of vitamin D in the etiopathogenesis of these diseases is not well understood. Similarly, the detailed mechanism of vitamin D action on thyroid hormone metabolism and autoimmune thyroid diseases is still poorly understood. It was recently reported that patients with autoimmune thyroid disease showed lower vitamin D levels. However, there are few studies dealing with vitamin D status in elderly patients with subclinical hypothyroidism. We therefore aimed to investigate vitamin D levels in elderly patients with subclinical hypothyroidism. In the present study, a total of 37 patients (>70 years old) with subclinical hypothyroidism and 40 healthy controls (>70 years old) were retrospectively analyzed. Serum levels of free triiodothyronine (fT3), free thyroxine (fT4) and thyroid-stimulating hormone (TSH) were analyzed by chemiluminescence immunoassay (Centaur XP, Siemens Healthcare Diagnostics Inc., Tarrytown, USA). Serum levels of 25-Hydroxy vitamin D (25-OH D) were measured by chemiluminescence immunoassay (DiaSorin, Liaison, Italy). Serum anti thyroid peroxidase (anti-TPO) and anti thyroglobulin (anti-TG) were measured using a solid-phase, enzyme-labeled, chemiluminenescent sequential immunometric assay (Immulite 2000 XPi, Siemens Healthcare Diagnostics Inc., Tarrytown, USA). There was no significant difference between groups in terms of age and gender distribution. Serum TSH, anti-TPO and anti-TG levels in elderly patients with subclinical hypothyroidism showed higher values than healthy controls as expected. Serum 25-OH D levels in elderly patients with subclinical hypothyroidism were lower than healthy controls (p<0.001). These data may suggest association between serum 25-OH D levels and subclinical hypothyroidism in elderly patients. Further studies are needed to determine whether vitamin D insufficiency is a casual factor in the etiopathogenesis of subclinical hypothyroidism in elderly patients or rather a consequence of the disease. © 2016 Elsevier B.V., All rights reserved.