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Öğe Association among ABCA7 Gene Polymorphism, rs3764650 and Alzheimer's Disease in the Turkish Population(Canadian Soc Clinical Investigation, 2015) Oznur, Murat; Hatipoglu, Omer F.; Ayturk, Zubeyde; Dede, Serap; Akbas, Kubra; Aydin, Duygu; Urhan, AysePurpose: Alzheimer's disease (AD), the most common form of dementia, is an irreversible and progressive neurodegenerative disease that is characterized by the progressive loss of cognitive functions, behavioral and psychological disorders and a decrease in daily routine activities. Among people aged 65 years and over, AD is steadily increasing. Genome-wide association studies have shown that various gene polymorphisms are highly associated with the pathogenesis of AD. Among them, ABCA7 gene polymorphism has been identified as one of the genetic risks. The purpose of this study was to investigate the relationship among ABCA7 gene polymorphism, rs3764650 and AD, and to determine if it could be use as a biomarker for AD susceptibility in the Turkish population. Methods: Peripheral blood samples of 54 Alzheimer's patients and 57 control subjects were collected. Genomic DNA was isolated by SDS/proteinase K treatment followed by phenolchloroform extraction and ethanol precipitation. The presence of the ABCA7 gene rs3764650 polymorphism was investigated by PCR-RFLP and selected samples were confirmed by DNA sequencing. Results: In the Turkish population, the ABCA7 gene rs3764650 polymorphism did not show a significant association with AD when compared with the control group (p>0.05). APOE-epsilon 3 allele frequencies were higher in both AD patients and control subjects (76.85% and 84.21%, respectively). Conclusion: Both previously published studies and our current study did not cover the complete genetic variation in the gene. To detect variants that are disease-related, studies with larger sample sizes are needed.Öğe Effects of Ginkgo biloba extract on brain oxidative condition after cisplatin exposure(Canadian Soc Clinical Investigation, 2016) Aydin, Duygu; Peker, Emine G. G.; Karakurt, Meryem D.; Gurel, Ayse; Ayyildiz, Mustafa; Cevher, Sule C.; Agar, ErdalPurpose: The purpose of this study was to evaluate the efficacy of Ginkgo biloba extract (EGb 761) on oxidative events of brain in cisplatin-administrated rats. Methods: Rats were divided into four experimental groups: 1) control (n=6); 2) cisplatin (8 mg/kg, intraperitoneally one dose, n=6); 3) EGb 761 (100 mg/kg intraperitoneally for 15 days, n=6); and 4) cisplatin + EGb 761 (n=6). After drug administration, rats were sacrificed and brain tissues were removed. Nitric oxide (NO), malondialdehyde (MDA) and glutathione (GSH) levels were evaluated in brain tissues. Results: Single dose cisplatin administration significantly increased NO and GSH levels, but decreased MDA levels in brain tissue samples. EGb 761 treatment reversed the effects of cisplatin on NO and GSH levels, but did not affect the decreased MDA levels. Conclusion: Results of the study indicate that oxidative stress can be an important pathogenetic mechanism of cisplatin-induced neurotoxicity. EGb 761, an standardized extract of G. biloba leaves that has antioxidant properties, may improve the oxidative stress-related neurological side effects of cisplatin.Öğe Potential beneficial effects of foot bathing on cardiac rhythm(Canadian Soc Clinical Investigation, 2016) Aydin, Duygu; Hartiningsih, Siti Sugih; Izgi, Melike Gokce; Bay, Sevgi; Unlu, Kubra; Tatar, Meryem Ozlem; Alparslan, Ayse MelikePurpose: Foot bathing therapy is a simple technique that induces sensations of comfort and relaxation. The aim of this study was to examine the effect of foot bathing therapy on heart rate variability (HRV) parameters in a healthy population. Methods: Participants were twenty healthy female subjects (median age= 20.67 years, SD= 1.04). The recording ECG was applied for 5 minutes before and for 5 minutes after foot bathing therapy of 10 minutes. Subjects rested for 10 minutes without recording ECG in order to stabilize autonomic parameters. The digital signals were then transferred to a laptop and analyzed using LabChart (R) software (MLS310/7 HRV Module). Results: Almost all HRV parameters increased and heart (pulse) rate and LF/HF ratio decreased after foot bathing therapy compared with before foot bathing therapy. Conclusions: These results indicate for the first time in humans that foot bathing might induce a state of balance between sympathetic and parasympathetic systems and might be helpful to prevent possible cardiac arrhythmias.Öğe Protective effect of melatonin on adriamycin-induced cardiotoxicity in rats(Aves, 2014) Bilginoglu, Ayca; Aydin, Duygu; Ozsoy, Seyma; Aygun, HaticeObjectives: Adriamycin is one of the most widely used anticancer drugs. The major limiting factor of using this drug is the development of cardiotoxicity. However, melatonin (N-acetyl-5- methoxytryptamine) is a ubiquitous molecule as a good antioxidant that may protect the heart. We investigated whether or not pretreatment with melatonin can attenuate adriamycin-induced cardiotoxicity. Study design: All procedures and experiments were approved by the Animal Ethics Committee of Gazi Osman Pasa University (2012-HADYEK-022). Adult male Wistar-Albino rats were randomly divided into four groups, namely control (CON, n=7), melatonin (MEL, n=7), adriamycin (ADR, n=7), and adriamycin+melatonin (ADR+MEL, n=7) groups. Cardiotoxicity in rats was induced by adriamycin injection (cumulative dose: 18 mg/kg, intraperitoneal [i.p.]) at an interval of 24 hours (h) on the 5th, 6th and 7th days. Rats receiving melatonin treatment in the adriamycin group received melatonin (10 mg/kg/day, i.p.) for 7 days and were injected with adriamycin (18 mg/kg, i. p.) on 5th, 6th and 7th days. On the 8th day, gravimetric, electrocardiography (ECG) and biochemical parameters were assessed. Results: Adriamycin induction caused changes in the ECG pattern, including ST-segment elevation and decreased R-amplitude, increase in the serum levels of cardiac injury markers (creatine kinase [CK], CK-MB, aspartate transaminase, and lactate dehydrogenase), decrease in the antioxidant enzymes activity (superoxide dismutase, glutathione peroxidase), elevated lipid peroxidation (malondialdehyde), and altered lipid profile in the serum. Melatonin treatment prevented all the parameters of adriamycin-induced cardiotoxicity in rats. Conclusion: Melatonin has a protective effect on the heart against adriamycin-induced cardiotoxicity in rats.Öğe Reflexological Therapy Induces a State of Balance in Autonomic Nervous System(Canadian Soc Clinical Investigation, 2015) Isik, Bunyamin; Aydin, Duygu; Arslan, Muzeyyen; Dane, SenolPurpose: Reflexology activate all body systems physically, mentally and emotionally by relieving pain and relaxing the body, in a manner similar to acupuncture and cupping therapies. The aim of this study was to examine the effect of reflexological therapy on heart rate variability (HRV) parameters in a healthy population. Methods: Participants were twenty-six healthy subjects (8 women, 18 men, median age=32.77 years, SD=8.04). All reflexology procedures were applied by a reflexology practitioner. The reflexology practitioner used thumbs and fingers to apply appropriate pressure to reflexology points, especially the heart point in both feet. The recording ECG was applied 1 hour before and 1 hour after reflexological therapy. Subjects rested for 10 minutes without recording ECG in order to stabilize autonomic parameters. The digital signals were then transferred to a laptop and analyzed using LabChart (R) software (MLS310/7 HRV Module). Results: Almost all HRV parameters increased and heart (pulse) rate and LF/HF ratio decreased after reflexological therapy compared with before reflexological therapy in healthy persons. Conclusions: These results indicate for the first time in humans that reflexology might induce a state of balance between sympathetic and parasympathetic systems and might be helpful to prevent possible cardiac arrhythmias. Therefore, reflexology may be accepted as a complementary therapy method for many cardiac problems, especially tachycardia and other cardiac arrhythmias.Öğe The acute and long term effects of acupuncture on heart rate variability(Natl Inst Science Communication-Niscair, 2015) Ozcan, Osman; Gunduz, Busra; Artut, Elif; Simbil, Kevser; Mansuroglu, Ayse Nazli; Akcaozoglu, Elif Beyza; Aydin, DuyguIn a recent study, the possible effect of acupuncture in modulating heart rate variability (HRV) in both healthy and non-healthy subjects were reported, while previous review reported that acupuncture did not have any convincing effect on HRV in healthy subjects. We aimed to reinvestigate the possible helpful effect of acupuncture on HRV parameters and to test the possible differences between acute and long term (20 minutes) acupuncture applications. Twenty nine female subjects were participated in this study. For the acupuncture therapy, HT 7 acupuncture point (shen men) was selected. Acupuncture needle was placed for 1 minute in acute acupuncture application and 20 minutes in long term acupuncture. The recording ECG (HRV) was applied before and after acute and long term acupuncture therapies. Almost all HRV parameters increased after the acute acupuncture and some HRV parameters after the long term acupuncture compared to before therapy in healthy persons. There were no differences between acute and long term applications. These results indicate that acupuncture might be cardio protective and might restore sympathovagal imbalances by stimulating the peripheral nervous system. Also, the long term acupuncture application is not essential for the cardio protective effect.Öğe The effect of combined treatment of alpha-tocopherol, ascorbic acid, and pyridoxine with NMDA blocker memantine on penicillin-induced epileptiform activity in rats(Tubitak Scientific & Technological Research Council Turkey, 2013) Aydin, Duygu; Yildirim, Mehmet; Ayyildiz, Mustafa; Agar, ErdalAim: To evaluate the effects of coadministration of vitamins alpha-tocopherol, ascorbic acid, and pyridoxine with memantine on a penicillin-induced experimental epilepsy model in rats so as to clarify the eventual interaction between these vitamins and the N-methyl-D-aspartate (NMDA) system. Materials and methods: The epileptic focus was produced by intracortical penicillin G potassium injection. The effects of intraperitoneal injections of memantine, memantine + alpha-tocopherol, memantine + ascorbic acid, and memantine + pyridoxine combinations on epileptiform activity were evaluated in electrocorticogram recordings. Results: The antiepileptiform effects appeared earlier in all memantine and vitamin coadministrated groups compared to the memantine-alone group, but the difference between the groups was not statistically significant considering the frequency and amplitude of the epileptiform activity. Conclusion: Coadministration of vitamins does not enhance the antiepileptiform activity of memantine in penicillin-induced epilepsy in rats. However, this coadministration causes the earlier appearance of antiseizure effects. Since moderate doses of these vitamins have no side effects, it might be a good idea to use them with NMDA blockers to provide an earlier antiepileptic effect.Öğe The Effect of Varying Doses of Intravenous Paracetamol on the Electrical Activity of the Brain in Penicillin-Induced Status Epilepticus in Rats(Kare Publ, 2015) Mumcuoglu, Ibrahim; Kurt, Semiha; Aydin, Duygu; Ekici, Fatih; Kasap, Zeynep; Solmaz, Volkan; Aygun, HaticeObjectives: Paracetamol is a widely used analgesic and antipyretic agent. It has been reported that N-arachidonoyl-phenolamine, the active metabolite of paracetamol, reduces epilepsy by activating the endocannabinoid system in some models of experimental epilepsy. Diazepam is a benzodiazepine well known to have anticonvulsant effects. The aim of the present study was to investigate the effects of different doses of paracetamol on penicillin-induced epileptiform activity (PIEA) in rats. Methods: Rats anesthetized with urethane (1.25 g/ kg, intraperitoneal) were placed in a stereotaxic frame. Body temperatures were maintained at 37 degrees C by a heating blanket. An epileptic focus was produced by 500 IU Penicillin G (PGP) injection into the soma-motor cortex using a hole drilled into the cranium. Paracetamol (100, 150 and 300 mg/ kg, respectively) and diazepam (5 mg/ kg) were administered thirty minutes after PGP injection, and their effects on the epileptiform activity were examined comparatively. Electrocorticographic activity was monitored for two hours. Results: Intracortical injection of PGP (500 units) induced epileptiform activity in all groups of rats. Diazepam caused a statistical significant decrease in the epileptiform activity in the 40th minute after PGP injection. Paracetamol (100 mg/ kg) application did not influence the PIEA (p> 0.05). However, 150 and 300 mg/ kg IV paracetamol had a statistically significant effect on the antiepileptic activity (p< 0.001). Conclusion: The results of the present study indicated that 150 and 300 mg/ kg doses of paracetamol had an effect on PIEA. Further studies are needed to understand the reasons for this effect.Öğe The effects of agomelatine and melatonin on ECoG activity of absence epilepsy model in WAG/Rij rats(Tubitak Scientific & Technological Research Council Turkey, 2015) Aygun, Hatice; Aydin, Duygu; Inanir, Sema; Ekici, Fatih; Ayyildiz, Mustafa; Agar, ErdalThe aim of this study was to evaluate the effect of the melatonergic M1 and M2 receptor agonist and serotonergic 5-HT2C receptor antagonist agomelatine on the spike wave discharges (SWDs) seen in electrocorticographic (ECoG) recordings of WAG/Rij rats with absence epilepsy. Twenty-one WAG/Rij male rats were used in this study. Tripolar electrodes were placed on skulls and control ECoG activities were recorded. Experimental groups received normal saline (Group I: 1 mL, intraperitoneally (i.p)), agomelatine (Group II: 40 mg/kg, i.p), and melatonin (Group III: 40 mg/kg, i.p) injections for 7 days. Following this period, 2-h ECoG recordings were repeated. The number of SWDs and their durations were calculated. The total number and duration of SWDs decreased in both the agomelatine and melatonin groups. The systemic administration of agomelatine and melatonin attenuated the genetic absence epilepsy seizures in WAG/Rij rats. The repressive effect of agomelatine on the absence seizures was similar to that of the melatonin used in this study.Öğe Wet Cupping Therapy Restores Sympathovagal Imbalances in Cardiac Rhythm(Mary Ann Liebert, Inc, 2014) Arslan, Muzeyyen; Yesilcam, Nesibe; Aydin, Duygu; Yuksel, Ramazan; Dane, SenolObjectives: A recent study showed that cupping had therapeutic effects in rats with myocardial infarction and cardiac arrhythmias. The current studyaimed to investigate the possible useful effects of cupping therapy on cardiac rhythm in terms of heart rate variability (HRV). Materials and methods: Forty healthy participants were included. Classic wet cupping therapy was applied on five points of the back. Recording electrocardiography (to determine HRV) was applied 1 hour before and 1 hour after cupping therapy. Results: All HRV parameters increased after cupping therapy compared with before cupping therapy in healthy persons. Conclusions: These results indicate for the first time in humans that cupping might be cardioprotective. In this study, cupping therapy restored sympathovagal imbalances by stimulating the peripheral nervous system.
